WebJan 1, 1999 · The reaction contained target (10 7 copies), 1 µM each primer, 10 mM Tris-HCl, pH 8.3, 50 mM KCl, 2.5 mM MgCl 2, 200 µM each dNTP and 2.5 U Taq polymerase … WebMar 16, 2024 · Commercially available 5′-acrydite-modified DNA primers are used to purify single stranded DNA (ssDNA) by immobilizing one strand of a PCR product within a polyacrylamide matrix. Single-stranded DNA …
Agardite - Wikipedia
WebDesigned primers bind only to the specific sequence flanking the sequence of interest in the genome that is amplified in the next step. In contrast, primers used for WGA bind to many places in the genome to start replication, thus the whole DNA strand is amplified. WebJan 1, 2024 · In MeRPy-PCR, deoxyuridine (dU) is site-specifically added to the acrydite-modified primer. After amplification, methanol purification, and denaturation of the mobile strand, the primer strand is recovered by uracil-DNA glycosylase (UDG) or dimethylethylenediamine (DMEDA). This method allows dual-purification of both the … high standards abatement
Modifications : Acrydite (Methacrylate) - biomers.net
Web2% acrylamide and the acrydite-modified DNA strands (1), 0.24 mM, and (3), 1.28 mM, was used for the polymerization. - Fort the system shown in Figure 4(A), consisting of domain A composed of two kinds of duplexes crosslinked hydrogel: A buffer solution (Tris-HCl, 10 mM, pH = 8.0), 200 µL, that WebAcrydite™ is an attachment chemistry based on an acrylic phosphoramidite that can be added to oligonucleotides as a 5’-modification. Acrydite-modified oligonucleotides … high speed tapered roller bearing