WebJan 1, 2024 · Rag2 mice (BALB/C) were crossed with Rag2/OT-I (C57BL/6) mice to generate F1 mice. Rag1 mice (C57BL/6) were crossed with 2C mice to generate the Rag1/2C mice. All mice were maintained in a specific-pathogen-free animal facility, and all experiments were conducted according to regulations of the Institutional Animal Care … WebC57BL/6-Tg (TcraTcrb)1100Mjb/J Stock No: 003831 Protocol 22848: Standard PCR Assay - Tg (Tcrb)1100Mjb Version 2. 3 Notes This assay will NOT distinguish hemizygous from …
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WebMay 12, 2024 · Female 6-12 week old C57Bl6J, Crl:NU-Foxn1 nu or C57BL/6-Tg (TcraTcrb)1100Mjb/J (OT-I) mice were purchased from Charles River (Saint-Germain-Nuelles, France; Calco, Italy). C57Bl6J mice for programmed cell death-1 (PD-1) blockade therapy were purchased from Harlan (Barcelona, Spain). ... Tumors were resected from … WebJan 15, 2024 · C57BL/6J and BALB/c mice were used as primary mouse T-cell donors for polyclonal T-cell expansion studies and C57BL/6-Tg(TcraTcrb)1100Mjb/J (OT-I) mice were used for antigen-specific T-cell ... red schoolhouse antiques millwood va
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WebMar 12, 2024 · OT-I mice: This TCR transgenic strain is also called C57BL/6-Tg (TcraTcrb)1100Mjb/J. We also highly recommend to cross OT-I to a congenic marker, such as B6.SJL-Ptprc a Pepc b /BoyJ (CD45.1 +), to track them in vivo upon adoptive transfer (see Note 7). 3. Batf3 −/− mice: Batf3-deficient mice is used as an excellent negative … WebOVA. (b) Percentage of OT-I cells (gated on live splenic CD45.1 +, CD44 +, and CD8a + cells) in C57BL/6 mice 3 days after injection of OT-I cells and cell-associated OVA 6. Please be aware that this strain is only applicable if your DC strain is C57BL/6. Alternatively, B6.129S2-Tap1. tm1Arp /J (Tap1 /) mice can be also used. 7. WebIn 1921, Littel obtained mice from Lathrop and named a strain of mice that was derived from the mating of female 57 and male 52 and that consistently exhibited black hair as … richwil facebook