Web025524 B6J.B6N(Cg)- Cx3cr1 tm1.1(cre)Jung /J Cx3cr1-Cre mice express Cre recombinase under the direction of the Cx3cr1 promoter in the mononuclear phagocyte system making them useful for fate-mapping studies of the monocyte and macrophage compartment, as well as microglia. These mice do not express endogenous Cx3cr1 . Web例如Christian Schulz等利用组成性表达Cre的Csf1r-cre鼠及诱导性表达Cre的Csf1r-MeriCreMer与Rosa26-LSL-YFP小鼠交配，获得在Csf1r基因表达的细胞中表达YFP荧光蛋白的小鼠，从而对Csf1r+细胞进行追踪，发现组织定居巨噬细胞起源于卵黄囊来源的Csf1r+细胞。

这些关于条件性基因敲除的问题你遇到了吗？ 南模生物

WebMacrophages are specialized phagocytes that display a variety of important functions for the host immune system. They are particularly important for the recognition of exogenous and endogenous danger signals, forming the defensive front line as part of innate immune response. As such, murine macroph … WebAug 7, 2024 · 2.1 新的Cre品系需要做的四件事：. 1) 了解小鼠来源，背景信息及发表的文章；. 2) 建立基因型鉴定方法，确认小鼠正确；. 3) 验证Cre在目标组织中的表达. 4) 验证Cre的敲除效率. 了解小鼠来源，背景信息及发表的文章. Mouse Genome Informatics CrePortal ：. 在Recombinase (cre)中 ... new smyrna beach water

Kidney‐specific knockout of Sav1 in the mouse promotes ...

WebAug 1, 2024 · Cre 工具鼠的种类及应用 Cre 工具鼠主要分为 3 类：全身性 Cre、组织特异性 Cre 和配体或药物诱导 Cre。 全身性 Cre：通过诱导剂来调节驱动 Cre 重组酶的启动子活 … WebAbstract. Ksp-cadherin is a unique, tissue-specific member of the cadherin family of cell adhesion molecules that is expressed in tubular epithelial cells in the kidney and developing genitourinary (GU) tract. It has recently been shown that a 1341-bp fragment of the 5' flanking region containing the Ksp-cadherin gene promoter can recapitulate ... Web今天，就和大家一起来了解下条件性基因敲除方法必用的Cre-loxP重组系统以及应用Cre-loxP进行条件性基因敲除的原则。. 概述. Cre-loxP重组系统，即对一段特定的DNA序列进行定位并用Cre重组酶对其进行剪接，由Cre重组酶和loxP位点两部分组成。. 其中Cre蛋白，最 … mid 20s identity crisis